THE EFFECT OF TOPICALLY USING MELATONIN AND ANABASIS ARTICULATE EXTRACT WITH VAGINAL SPONGES ON SOME HORMONES AND ANTIOXIDANT STATUS

This study was conducted in the University of Anbar College Agriculture Department Animal Production Sheep Field, for period from 10/15/2021 to 5/7/2022. The aim this is use melatonin hormone two different doses and Anabasis articulate extract vaginal sponges topically, understand their effectiveness on oxidative state some reproductive hormones local Iraqi ewes. twenty ewes within 4-2.5 years age range 47 kg weight average. All were treated with 14 days, randomly divided into four treatment groups: (T1) 20 mg hormone, (T2) 30mg (T3) at a concentration 3 g (T4) left without any considered control treatment. applied through sponge hole made it using surgical scalpel; that position should be away pulling thread attached avoid cutting accidentally thus not being able pull out. continued day 0 pushing until when they withdrawn. injected eCG dose 500 IU intramuscularly after withdrawal. Also, experimental kept rams days. Blood samples collected jugular vein levels estrogen, progesterone, antioxidants glutathione peroxides (GPX), Malone Dialdehyde (MDA) measured. experiment treatments showed no significant differences both estrogen progesterone compared group, which indicates there effect materials absorption. As antioxidants, superiority GSH group. MDA recorded decrease its groups one. We conclude placing 20,30 Mg 3g did affect hormone’s actions neither absorption sponge, but rather improved uterus conditions prevent fluid accumulation around sponge. It also prevents infections improves oxidation by increasing activity level reduction.